Core Facility

The BD FACSAria III sorter is a superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve unrivaled sensitivity and resolution. BD FACSAria III cell sorter with a 5 laser 11 colour setup. The BD FACSAria III system has a throughput of 70,000 events per second and can do 4-way sorting and single cell sorting. Wavelength choices include 561-nm (Y/G), as well as the 488-nm (Blue), 633-nm (Red), 405-nm (Violet), and 375-nm (UV) lasers.

BD FACS celesta is a multi-laser flow cytometer with 3 laser and 12 colour setup for delivering high sensitivity and performance. All configurations have blue (488-nm) and violet (405-nm) lasers, which has been paired with a yellow-green (561-nm) laser, for your application needs.

The High performance BC CytoFLEX-LX Flow cytometer analyser with Five High Power Lasers 488nm, 638nm, 405nm, 561nm and 355nm, 19 Colors & 21 Parameters (R3 B3-V5-YG5–UV3 System) is used for qualitative and quantitative measurement of biological and physical properties of cells and other particles. The system offers the ability to configure the violet laser detector (VSSC) to collect side scatter to better resolve nanoparticles from noise. It has Superior Acquisition rate of 30,000 events per second and Superior Signal Processing digital system with 7 decades dynamic range.

The Leica DMI6000B is an inverted fluorescence microscope comprising of 6 interchangeable filters for detecting various fluorochromes. It has two independent cameras – DFC295 for high resolution bright-field imaging and DFC360 FX for high frame rate fluorescence imaging. It is also equipped with a fluorescence intensity manager and programmable function keys for easy access to functions.

The EVOS® FL Auto Imaging System is a fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system with outstanding workflow efficiency. Designed to meet demanding requirements over a broad range of applications, the EVOS® FL Auto system supports high-resolution mosaic tiling, multi-position well scanning, cell counting with thresholding, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage and parfocal optical system enables us to produce publication quality images in seconds. The EVOS® FL Auto system can be programmed to run acquisition routines, 8-point time lapse experiments, and tile-stitch scans in nearly any vessel through the sensitive touch-screen display.

Olympus BX43F upright microscope, Leica DMIL (upright) and Leica DMI1000 (inverted) microscopes are available for users to perform routine light microscopy imaging. DMIL and DMI1000 microscopes are provided with an interchangeable Leica DFC290 camera for high resolution bright-field imaging. The Leica DMI1000 is also installed in the tissue culture facilities of individual labs and the Core tissue culture area.

The Olympus FV1000 confocal system comprises a motorized microscope with z focus drift compensation facility for bright field, differential interference contrast and fluorescence imaging with motorized XY scanning stage and CO2 incubation facility for live cell imaging. It is equipped with the following lasers - 405nm, Multi-Argon (458nm, 488nm and 515nm), 559nm and 635nm. Apart from regular confocal imaging, this microscope can be used to perform Multi-Area Time Lapse, FRET, FRAP, FLIM and diffusion experiments.

The FV1000MPE is an upright multiphoton laser-scanning microscope coupled with a Mai Tai HP-Deep See–OL laser with automated broadband wavelength tuning from 690 to 1040nm for deep tissue imaging.

The systems supports multicolor fluorescent studies for   imaging of living, whole mount or thickly sliced specimen.  Dynamic biological processes can be imaged hundreds of micrometers within living cells and tissues. Provides support for applications where phototoxicity/photobleaching are a concern such as time course studies of living cells and tissues. Low magnification lens and long working distance stage allow imaging of large samples, embryos, and animals.

The Imaging Core Facility conducts training sessions regularly for both first time and experienced users. The training sessions comprise of specifically designed modules which include theory and practical sessions. The final authorization is given to the user upon successfully completing the required modules. The hands-on training sessions are tailored to the specific application requirement of each user so that they get the maximum benefit out of these systems. Apart from in-house training, the imaging core organizes sessions by application specialists from Leica and Olympus.

Genetic Analyzer 3130 is a 4 capillary series system with Electroosmotic flow suppression polymers (EOF). This system gives you all the advance automation with hands free operation and superior performance. This system provides compatibility with the existing application software systems, long-term reliability, automated polymer delivery system, enhanced thermal control, and optimized for multiple application.

QuantStudio 12 K Flex system is designed for maximum throughput, outstanding flexibility with 5-interchangeable blocks, scalability and user friendly. This system is widely used in gene expression analysis, SNP genotyping, copynumber analysis, digital PCR technology, Micro RNA and other noncoding RNA analysis.
QuantStudio 6 K Flex system facilitates high-throughput analysis with 2-interchangeable blocks. This system is commonly used for PCR applications such as gene expression, gene regulation, gene variation and protein expression.

The Radioactivity Core Facility provides researchers a secure access to radiolabelled isotopes and instrumentation for detecting radioactivity. The facility currently has Greiger counters, GE Storm 365 Phosphor imager and a Perkin Elmer Tricarb Liquid Scintillation Counter.

Alcian Blue, Perl’s Prussian Blue Iron Stain, Periodic Acid Schiff, Masson Trichrome, Gordon Sweet Reticulin, Acid Fast Bacillus stain for Mycobacterium Tuberculosis, Toluidine Blue, Masson Fontana, Verhoeff’s elastic stain, Sirus Red stain, Hematoxylin and Eosin (H&E), Safaranin-O, Elastic van Gieson, Paraffin blocks and tissue mounting, Unstained slide sections for specific immunostaining.

Cytospin preparation, Cell block preparation, Cryostate sectioning.